ftsW [2018-02-12T20:16:19.000Z]

SEDS family peptidoglycan glycosyltransferase, cell-division protein

locus: BSU14850

pI: 9.72

mw: 43.57 kDa

protein length: 403 aa

gene length: 1209 bp

function: cell division

product: SEDS family peptidoglycan glycosyltransferase

essential: yes

synonyms: ylaO

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,552,899 → 1,554,110

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• principal peptidoglycan glycosyltransferase of the [SW|divisome] [Pubmed|27525505]

Protein family

• [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW]/[protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA]/[protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE] family (according to Swiss-Prot)
• [SW|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]

Paralogous protein(s)

• [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA], [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE]

[SW|Localization]

• integral membrane protein
• localizes to the division site [Pubmed|27895631]
• localization depends on [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] [Pubmed|26679002]

Expression and Regulation

Operons

• genes: [gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]-[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]
  description: [pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|YofA]: activation, [Pubmed|17526699], in [regulon|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|YofA regulon]
  • [regulon|stringent response|stringent response]: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • constitutively expressed [Pubmed|23701187]

Biological materials

Mutant

• MGNA-A563 (ftsW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/563 NBRP B. subtilis, Japan]

FLAG-tag construct

• GP2007 ''ftsW-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 26679002,28975672

Original Publications

• 17981970,19429628,17526699,21564336,23701187,27525505,27895631,17379809,27895631